上海酶聯(lián)為您詳解白細胞介素2試劑盒的生物學活性測定

<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">上海酶聯(lián)生物科技有限公司專業(yè)代理人白介素</font>elisa<font face="宋體">試劑盒，大鼠白介素</font><font face="Calibri">Elisa</font><font face="宋體">試劑盒，小鼠</font><font face="Calibri">Elisa</font><font face="宋體">試劑盒等多類種屬，歡迎來電咨詢第二季度優(yōu)惠價格。我司提供免費技術(shù)指導及代測服務，為您的實驗帶來全方位的服務體驗。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="color: rgb(255, 0, 0);"><span style="font-family: 宋體; font-size: 9pt;">★&nbsp;實驗目的</span></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">①</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"> </span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">掌握</font>IL-2<font face="宋體">生物學活性測定的原理。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">②</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"> </span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">熟悉</font>IL-2<font face="宋體">生物學活性測定的實驗方法和結(jié)果計算。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="color: rgb(255, 0, 0);"><span style="font-family: 宋體; font-size: 9pt;">★</span><span style="font-family: 宋體; font-size: 9pt;">&nbsp;</span><span style="font-family: 宋體; font-size: 9pt;">實驗原理</span></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">IL-2<font face="宋體">是由</font><font face="Calibri">Th</font><font face="宋體">細胞產(chǎn)生的淋巴因子，在淋巴細胞增殖分化過程中起到非常重要的作用。</font><font face="Calibri">IL-2</font><font face="宋體">活性測定基于</font><font face="Calibri">IL-2</font><font face="宋體">能維持</font><font face="Calibri">IL-2</font><font face="宋體">依賴細胞的代謝和存活，促進這類細胞的增殖。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">細胞在增殖時能量代謝活躍，可產(chǎn)生大量的能量以供合成多種大分子物質(zhì)和細胞分裂所需，能量代謝的水平與細胞合成</font>DNA<font face="宋體">水平基本平行，因此，測定細胞能量代謝的水平可以間接地反映細胞增殖情況。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">MTT<font face="宋體">（四甲基偶氮唑鹽）是一種淡黃色的水溶性化合物，活細胞（特別是增殖期的細胞）通過線粒體能量代謝過程中的琥珀酸脫氫酶的作用，使淡黃色的</font><font face="Calibri">MTT</font><font face="宋體">分解產(chǎn)生藍色結(jié)晶狀甲臢沉積于細胞內(nèi)或細胞周圍，且形成甲臢的量與細胞增殖程度呈正比，甲臢經(jīng)</font><font face="Calibri">SDS</font><font face="宋體">作用后可溶解顯色。溶解液的光密度值與細胞代謝及</font><font face="Calibri">IL-2</font><font face="宋體">活性正相關。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="color: rgb(255, 0, 0);"><span style="font-family: 宋體; font-size: 9pt;">★</span><span style="font-family: 宋體; font-size: 9pt;">&nbsp;</span><span style="font-family: 宋體; font-size: 9pt;">實驗材料</span></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">1.</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">1640<font face="宋體">培養(yǎng)液（完全）、</font><font face="Calibri">IL-2</font><font face="宋體">標準品、待測</font><font face="Calibri">IL-2</font><font face="宋體">樣品、</font><font face="Calibri">CTLL-2</font><font face="宋體">細胞株、</font><font face="Calibri">MTT</font><font face="宋體">溶液（</font><font face="Calibri">5mg/ml</font><font face="宋體">）、</font><font face="Calibri">10%SDS</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">2.</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">96<font face="宋體">孔細胞培養(yǎng)板、多頭細胞收集器、微量加樣器、</font><font face="Calibri">CO2</font><font face="宋體">孵箱、酶標儀</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="color: rgb(255, 0, 0);"><span style="font-family: 宋體; font-size: 9pt;">★</span><span style="font-family: 宋體; font-size: 9pt;">&nbsp;</span><span style="font-family: 宋體; font-size: 9pt;">實驗方法</span></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">①</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"> </span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">制備</font>CTLL-2<font face="宋體">細胞懸液</font><font face="Calibri">&nbsp; </font><font face="宋體">取生長旺盛的</font><font face="Calibri">CTLL-2</font><font face="宋體">細胞，用</font><font face="Calibri">1640</font><font face="宋體">培養(yǎng)液將細胞洗</font><font face="Calibri">3</font><font face="宋體">次，用完全</font><font face="Calibri">1640</font><font face="宋體">培養(yǎng)液配成</font><font face="Calibri">2</font><font face="宋體">&times;</font><font face="Calibri">105/ml</font><font face="宋體">細胞懸液。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">②</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"> </span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">稀釋樣品和標準品</font>&nbsp; <font face="宋體">將待測樣品和標準</font><font face="Calibri">IL-2</font><font face="宋體">用完全</font><font face="Calibri">1640</font><font face="宋體">培養(yǎng)液做一定的倍比稀釋。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">③</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"> </span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">加樣與檢測</font>&nbsp; <font face="宋體">向</font><font face="Calibri">96</font><font face="宋體">孔細胞培養(yǎng)板內(nèi)加入不同稀釋度的樣品和標準品（</font><font face="Calibri">50</font><font face="宋體">&mu;</font><font face="Calibri">l/</font><font face="宋體">孔），每稀釋度</font><font face="Calibri">3</font><font face="宋體">個復孔，并設細胞對照。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">再向各孔加入</font>50<font face="宋體">&mu;</font><font face="Calibri">l</font><font face="宋體">細胞懸液，混勻，置</font><font face="Calibri">5%CO237</font><font face="宋體">℃培養(yǎng)</font><font face="Calibri">36</font><font face="宋體">小時，每孔加入</font><font face="Calibri">MTT</font><font face="宋體">溶液</font><font face="Calibri">20</font><font face="宋體">&mu;</font><font face="Calibri">l CO2</font><font face="宋體">孵育</font><font face="Calibri">6</font><font face="宋體">～</font><font face="Calibri">8</font><font face="宋體">小時后，每孔再加</font><font face="Calibri">10%SDS100</font><font face="宋體">&mu;</font><font face="Calibri">l</font><font face="宋體">，充分混勻，</font><font face="Calibri">37</font><font face="宋體">℃孵箱靜放（使甲臢全溶解）。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">在酶聯(lián)儀上選波長</font>570nm<font face="宋體">測定</font><font face="Calibri">OD</font><font face="宋體">值，將待測樣品的</font><font face="Calibri">OD</font><font face="宋體">值與標準品</font><font face="Calibri">OD</font><font face="宋體">值比較后，求得待測樣品的</font><font face="Calibri">IL-2</font><font face="宋體">活性單位。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="color: rgb(255, 0, 0);"><span style="font-family: 宋體; font-size: 9pt;">★</span><span style="font-family: 宋體; font-size: 9pt;">&nbsp;</span><span style="font-family: 宋體; font-size: 9pt;">結(jié)果計算</span></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">將各稀釋度的</font>OD<font face="宋體">值按照樣品增殖</font><font face="Calibri">OD</font><font face="宋體">值的百分比換算成概率單位，可將原來呈</font><font face="Calibri">S</font><font face="宋體">形的曲線轉(zhuǎn)換成為直線。根據(jù)這些點的數(shù)據(jù)求出各直線的回歸方程。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">再從回歸方程求出各樣品達</font>50%*<font face="宋體">增殖時的稀釋度，而后按下列公式求出待測樣品</font><font face="Calibri">IL-2</font><font face="宋體">的活性單位。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="color: rgb(255, 0, 0);"><span style="font-family: 宋體; font-size: 9pt;">★</span><span style="font-family: 宋體; font-size: 9pt;">&nbsp;</span><span style="font-family: 宋體; font-size: 9pt;">計算公式：</span></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;x&nbsp; </span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">待測樣品</font><font face="Calibri">IL-2</font><font face="宋體">活性單位（&mu;</font><font face="Calibri">/ml</font><font face="宋體">）；</font><font face="Calibri">&nbsp; a</font><font face="宋體">：標準參考樣品</font><font face="Calibri">IL-2</font><font face="宋體">的活性單位（&mu;</font><font face="Calibri">/ml</font><font face="宋體">）；</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;d&nbsp; </span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">待測樣品達</font><font face="Calibri">50%*</font><font face="宋體">增殖的稀釋度；</font><font face="Calibri">&nbsp; D</font><font face="宋體">：標準參考樣品在</font><font face="Calibri">50%*</font><font face="宋體">增殖的稀釋度。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="color: rgb(255, 0, 0);"><span style="font-family: 宋體; font-size: 9pt;">★</span><span style="font-family: 宋體; font-size: 9pt;">&nbsp;</span><span style="font-family: 宋體; font-size: 9pt;">注意事項</span></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">1<font face="宋體">．</font><font face="Calibri">&nbsp; </font></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="Calibri">MTT</font><font face="宋體">液要現(xiàn)配現(xiàn)用，避免光照，若有藍色顆粒需過濾后再用。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">2<font face="宋體">．</font></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="Calibri">&nbsp; </font><font face="宋體">生物學測定法敏感性高，特異性強，所測</font><font face="Calibri">IL-2</font><font face="宋體">是具有生物活性的</font><font face="Calibri">IL-2</font><font face="宋體">，而不象免疫學測定法所測定免疫反應性</font><font face="Calibri">IL-2</font><font face="宋體">蛋白，因此測定的條件和要求要嚴格按規(guī)程。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>